Residue Analysis of Oxytetracycline in Milk Sample by Two Different Chromatographic Methods and Determination of Ionization Constant Values

In thepresent study, two analytical methods for the residue analysis of oxytetracycline in milk sample have been generated. InHPLC method, the analysis was performed on an X Terra RP-18 column at 25 °C with the mobile phase as methanol: water (20 : 80 )(v/v) modified to pH 5. For the second method capillary electrophoresis system was used. The analysis of oxytetracycline in milk sample could be achieved without using organic modifier in a 58 cm length capillary at a working voltage of 12 kV with 20 mM NaH2PO4-H3PO4(pH 7) by capillary electrophoresis. Tetracycline was used as internal standard in both methods. The results calculatedfrom both methods were compared to each other. The calculated data for drugs was checked with the data predicted by the SPARC on-line pKa estimator

A Novel and Rapid HPLC Method for Determination of Natamycin in Turkish Cheese Samples

Nicotine is a potential inducer of oxidative stress, which can damage the numerous tissues and biological molecules.Nicotine is first metabolized in liver, and the lung is the main target organ susceptible to smoking; therefore, the purpose ofthe current examination was scrutinizing the impacts of nicotine on these organs using the histological method, and studyingthe ameliorative (additive or synergistic) effects of quercetin and luteolin supplements in target tissues against nicotinetoxicity in young albino rats. Fifty animals were involved in this study which were divided into five groups of ten rats, theywere treated as: (1) untreated Control (Cont); (2) Nicotine (Ni) treated (0.75 mg/kg b.w./d, i.p.); (3) Nicotine as above withintragastric administration of quercetin (Ni+Qu) (50mg/kg b.w) or (4) with luteolin (50mg/kg b.w) in Group (Ni+Lu), and(5) nicotine with combination of quercetin and luteolin (Ni+Qu+Lu) as described above. All groups were treated for 8weeks, then their liver and lung tissues were dissected out. Several histological damages were noticed in (Ni) rats such asdegeneration changes in hepatocytes, slight congestion of central vein (CV) and cellular infiltration. Nicotine caused drasticchanges in lung tissues such as, inflammatory cells aggregation inside and outside the bronchiol artery with mild increase inthe thickness of bronchial muscular wall and marked thickening in the alveolar wall. All supplemented groups amelioratedthe damage that induced by nicotine injection in both liver and lung tissues. In liver tissues, the quercetin (Ni+Qu)supplemented rats showed the better improvement nearby normal hepatocytes than the other groups, while in lung tissues,the luteolin supplemented rats had better recovery than the other treatments. These observations suggested that the intake ofquercetin and luteolin as supplements may be useful in combating tissue injury that is a result of nicotine toxicity